| Team | Owner | Farm system | Stadium |
|---|---|---|---|
| Arizona Flyers | Mark Zebehazy | Houston Astros | Miller Park |
| Bakersfield Train Robbers | Brandt Ricker | Oakland A's | Target Field |
| California Ducks | Casey Painter | Baltimore Orioles | Progressive Field |
| Detroit Falcons | Brian McDaniels | Detroit Tigers | Comerica Park |
| Hudson Valley Canolies | Rich Molino | New York Mets | Citi Park |
| Iowa Cubs | Devin Ricker | Chicago Cubs | [2025] PNC Park |
| Kansas City Monarchs | Michael Ochinero | Tampa Bay Rays | [2025] Oracle Park |
| Madison Muskies | Daryl Spors | Cincinnati Reds | Angel Stadium |
| Minnow Lake Lynx | Yvan Roy | Cleveland Guardians | Wrigley Field |
| New York Underdogs | Seth Greenspan | Los Angeles Dodgers | Yankee Stadium |
| Pittsburgh Crawfords | Steve Lockney | New York Yankees [2026] San Diego Padres | Petco Park |
| Portland Mavericks | Chet Porowski | St. Louis Cardinals | Camdem Yards |
| Portsmouth Pirates | Chuck Carline | Arizona Diamondbacks | Fenway Park |
| Seattle StormBringer | Nate Pasilis | Seattle Mariners | Safeco Field |
| Sudbury Black Sox | Mickey Teed | Boston Red Sox | Kaufman Stadium |
| Tucson Desert Rats | Michael Schutt | Atlanta Braves | Chase Field |